Publications from Shmulevitz lab (July 2011 - now)
Kniert J.*, Dos Santos T.*, Joyce M., Shmulevitz M. (2022) Spatial compartmentalization of reovirus core versus full virus assembly. PLOS Pathogens 18(9): e1010641. https://doi.org/10.1371/journal.ppat.1010641
Cristi F, Gutierrez T, Hitt MM, Shmulevitz M. (2022) Genetic Modifications That Expand Oncolytic Virus Potency. Front Mol. Biosci. Jan 26;9:831091. doi: 10.3389/fmolb.2022.831091.
Yip WK, Cristi F, Trifonov G, Narayan N, Kubanski M, Shmulevitz M. (2021) The reovirus µ2 C-terminal loop inversely regulates NTPase and transcription functions versus binding to factory-forming µNS and promotes replication in tumorigenic cells. J Virol. Mar 3:JVI.02006-20. doi: 10.1128/JVI.02006-20.
Kniert J., Lin WR., Shmulevitz M. (2021) Captivating Perplexities of Spinareovirinae 50 RNA Caps. Viruses Feb 13;13(2):294. doi: 10.3390/v13020294.
Mohamed A, Konda P, Eaton HE, Gujar S, Smiley JR, Shmulevitz M. (2020) Closely related reovirus lab strains induce opposite expression of RIG-I/IFN-dependent versus -independent host genes, via mechanisms of slow replication versus polymorphisms in dsRNA binding σ3 respectively. PLoS Pathog. Sep 21;16(9) doi: 10.1371/journal.ppat.1008803.
Mohamed A, Smiley JR, Shmulevitz M. (2020) Polymorphisms in the Most Oncolytic Reovirus Strain Confer Enhanced Cell Attachment, Transcription, and Single-Step Replication Kinetics. J Virol. Jan 31;94(4). doi: 10.1128/JVI.01937-19. (Chosen for spotlight DOI: 10.1128/JVI.02070-19)
Mohamed A, Clements DR, Gujar SA, Lee PW, Smiley JR, Shmulevitz M. Single Amino Acid Differences between Closely Related Reovirus T3D Lab Strains Alter Oncolytic Potency In Vitro and In Vivo (2020). J Virol. Jan 31;94(4). doi: 10.1128/JVI.01688-19. (Chosen for spotlight DOI: 10.1128/JVI.02070-19)
Philip A., Perry J., Eaton H., Shmuleitz M., Hyser, JH., Patton, J. (2019) Generation of Recombinant Rotavirus Expressing NSP3-UnaG Fusion Protein by a Simplified Reverse Genetics System. J Virol. 2019 Oct 9. pii: JVI.01616-19. doi: 10.1128/JVI.01616-19.
Fernandes PJ., Cristi F., Eaton HE, Chen P., Haeflinger S., Bernard I., Hitt MM., Shmulevitz M. (2019) Breast tumor-associated metalloproteases restrict reovirus oncolysis by cleaving the σ1 cell-attachment protein, and can be overcome by mutation of σ1. J Virol. Aug 28. 93(2) pii: JVI.01380-19. doi:10.1128/JVI.01380-19. (Chosen for spotlight DOI: 10.1128/JVI.01548-19)
Jaïs, PH., Decroly, E., Jacquet, E., Le Boulch, M., Jaïs, A., Jean-Jean, O., Eaton, H., Ponien, P., Verdier, F., Canard, B., Goncalves, S., Chiron, S., Le Gall, M., Mayeux, P., Shmulevitz, M. (2019) C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system. Nucleic Acids Res. Mar 18;47(5):2681-2698. doi: 10.1093/nar/gkz069.
Bourhill, T., Mori, Y., Rancourt, D.E., Shmulevitz, M., Johnston, R.N. (2018) Going (reo) Virol: Factors Promoting Successful Reoviral Oncolytic Infection. Viruses, 10(8), 421-447; doi:10.3390/v10080421
Eaton, H., Dermody, T., Johnston, R., Jais, P., Shmulevitz, M. (2017) African swine fever virus NP868R capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and RNA incorporation into infectious virions. J. Virology May 12;91(11). pii: e02416-16. doi: 10.1128/JVI.02416-16. (Chosen for spotlight DOI: 10.1128/JVI.00566-17)
James, KT, Cooney, B., Agopsowicz K., Trevors, MA, Mohamed, A, Stoltz, D., Hitt, M., Shmulevitz M (2016) High-throughput Approach for Purification of Infectious Virions. Scientific Reports Nov 9;6:36826. doi: 10.1038/srep36826.
Mohamed A, Johnston R, Shmulevitz, M. (2015). Potential for Improving Potency and Specificity of Reovirus Oncolysis with Next-Generation Reovirus Variants. Viruses (Review) Dec 1;7(12):6251-78. doi: 10.3390/v7122936
Shmulevitz M and Mohamed A. (2015) Nogo to IFN by Ras. Cell Cycle (Invited News and Views). Aug 3;14(15):2392-3. doi: 10.1080/15384101.2015.1063310.
Garant K, Shmulevitz M, Pan L, Daigle R, Ahn DG, Gujar S, Lee PW. (2015) "Oncolytic reovirus induces intracellular redistribution of Ras to promote apoptosis and progeny virus release. Oncogene May 11. doi: 10.1038/onc.2015.136.
Mohamed A, Teicher C, Haefliger S, Shmulevitz M. (2015) Reduced virion-associated σ1 fibers on oncolytic reovirus variants promotes adaptation towards tumorigenic cells. J Virol. 89(8): 4319-4334. doi: 10.1128/JVI.03651-14
Cooper T, Biron B, Fast D, Tam R, Carey T, Shmulevitz, M*, Seikaly H* (2015) Oncolytic activity of reovirus in HPV positive and negative head and neck squamous cell carcinoma. J Otolaryngol Head Neck Surg. 2015 Feb 24;44:8. doi: 10.1038/onc.2015.136. (*corresponding authors)
Pan D, Marcato P, GyunAhn D, Gujar S, Pan LZ, Shmulevitz M, Lee PWL. (2013) Activation of p53 by Chemotherapeutic Agents Enhances Reovirus Oncolysis. PLOS ONE, 8 (1).
Gujar SA, Dielschneider R, Clements D, Helson E, Shmulevitz M, Marcato P, Pan D, Pan L, Alawadh A, Lee PWL. (2013) Multifaceted Therapeutic Targeting of Ovarian Peritoneal Carcinomatosis through Virus induced Immuno-modulation. Mol. Ther., 2:338-47.
Shmulevitz M, Gujar SA, GyunAhn D, Mohamed, A., Lee PWL. (2012) Reovirus variants with mutations in S1 and L2 genome segments exhibit enhanced virion infectivity and superior oncolysis. J. Virol., 86(13):7403-13.
Cristi F, Gutierrez T, Hitt MM, Shmulevitz M. (2022) Genetic Modifications That Expand Oncolytic Virus Potency. Front Mol. Biosci. Jan 26;9:831091. doi: 10.3389/fmolb.2022.831091.
Yip WK, Cristi F, Trifonov G, Narayan N, Kubanski M, Shmulevitz M. (2021) The reovirus µ2 C-terminal loop inversely regulates NTPase and transcription functions versus binding to factory-forming µNS and promotes replication in tumorigenic cells. J Virol. Mar 3:JVI.02006-20. doi: 10.1128/JVI.02006-20.
Kniert J., Lin WR., Shmulevitz M. (2021) Captivating Perplexities of Spinareovirinae 50 RNA Caps. Viruses Feb 13;13(2):294. doi: 10.3390/v13020294.
Mohamed A, Konda P, Eaton HE, Gujar S, Smiley JR, Shmulevitz M. (2020) Closely related reovirus lab strains induce opposite expression of RIG-I/IFN-dependent versus -independent host genes, via mechanisms of slow replication versus polymorphisms in dsRNA binding σ3 respectively. PLoS Pathog. Sep 21;16(9) doi: 10.1371/journal.ppat.1008803.
Mohamed A, Smiley JR, Shmulevitz M. (2020) Polymorphisms in the Most Oncolytic Reovirus Strain Confer Enhanced Cell Attachment, Transcription, and Single-Step Replication Kinetics. J Virol. Jan 31;94(4). doi: 10.1128/JVI.01937-19. (Chosen for spotlight DOI: 10.1128/JVI.02070-19)
Mohamed A, Clements DR, Gujar SA, Lee PW, Smiley JR, Shmulevitz M. Single Amino Acid Differences between Closely Related Reovirus T3D Lab Strains Alter Oncolytic Potency In Vitro and In Vivo (2020). J Virol. Jan 31;94(4). doi: 10.1128/JVI.01688-19. (Chosen for spotlight DOI: 10.1128/JVI.02070-19)
Philip A., Perry J., Eaton H., Shmuleitz M., Hyser, JH., Patton, J. (2019) Generation of Recombinant Rotavirus Expressing NSP3-UnaG Fusion Protein by a Simplified Reverse Genetics System. J Virol. 2019 Oct 9. pii: JVI.01616-19. doi: 10.1128/JVI.01616-19.
Fernandes PJ., Cristi F., Eaton HE, Chen P., Haeflinger S., Bernard I., Hitt MM., Shmulevitz M. (2019) Breast tumor-associated metalloproteases restrict reovirus oncolysis by cleaving the σ1 cell-attachment protein, and can be overcome by mutation of σ1. J Virol. Aug 28. 93(2) pii: JVI.01380-19. doi:10.1128/JVI.01380-19. (Chosen for spotlight DOI: 10.1128/JVI.01548-19)
Jaïs, PH., Decroly, E., Jacquet, E., Le Boulch, M., Jaïs, A., Jean-Jean, O., Eaton, H., Ponien, P., Verdier, F., Canard, B., Goncalves, S., Chiron, S., Le Gall, M., Mayeux, P., Shmulevitz, M. (2019) C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system. Nucleic Acids Res. Mar 18;47(5):2681-2698. doi: 10.1093/nar/gkz069.
Bourhill, T., Mori, Y., Rancourt, D.E., Shmulevitz, M., Johnston, R.N. (2018) Going (reo) Virol: Factors Promoting Successful Reoviral Oncolytic Infection. Viruses, 10(8), 421-447; doi:10.3390/v10080421
Eaton, H., Dermody, T., Johnston, R., Jais, P., Shmulevitz, M. (2017) African swine fever virus NP868R capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and RNA incorporation into infectious virions. J. Virology May 12;91(11). pii: e02416-16. doi: 10.1128/JVI.02416-16. (Chosen for spotlight DOI: 10.1128/JVI.00566-17)
James, KT, Cooney, B., Agopsowicz K., Trevors, MA, Mohamed, A, Stoltz, D., Hitt, M., Shmulevitz M (2016) High-throughput Approach for Purification of Infectious Virions. Scientific Reports Nov 9;6:36826. doi: 10.1038/srep36826.
Mohamed A, Johnston R, Shmulevitz, M. (2015). Potential for Improving Potency and Specificity of Reovirus Oncolysis with Next-Generation Reovirus Variants. Viruses (Review) Dec 1;7(12):6251-78. doi: 10.3390/v7122936
Shmulevitz M and Mohamed A. (2015) Nogo to IFN by Ras. Cell Cycle (Invited News and Views). Aug 3;14(15):2392-3. doi: 10.1080/15384101.2015.1063310.
Garant K, Shmulevitz M, Pan L, Daigle R, Ahn DG, Gujar S, Lee PW. (2015) "Oncolytic reovirus induces intracellular redistribution of Ras to promote apoptosis and progeny virus release. Oncogene May 11. doi: 10.1038/onc.2015.136.
Mohamed A, Teicher C, Haefliger S, Shmulevitz M. (2015) Reduced virion-associated σ1 fibers on oncolytic reovirus variants promotes adaptation towards tumorigenic cells. J Virol. 89(8): 4319-4334. doi: 10.1128/JVI.03651-14
Cooper T, Biron B, Fast D, Tam R, Carey T, Shmulevitz, M*, Seikaly H* (2015) Oncolytic activity of reovirus in HPV positive and negative head and neck squamous cell carcinoma. J Otolaryngol Head Neck Surg. 2015 Feb 24;44:8. doi: 10.1038/onc.2015.136. (*corresponding authors)
Pan D, Marcato P, GyunAhn D, Gujar S, Pan LZ, Shmulevitz M, Lee PWL. (2013) Activation of p53 by Chemotherapeutic Agents Enhances Reovirus Oncolysis. PLOS ONE, 8 (1).
Gujar SA, Dielschneider R, Clements D, Helson E, Shmulevitz M, Marcato P, Pan D, Pan L, Alawadh A, Lee PWL. (2013) Multifaceted Therapeutic Targeting of Ovarian Peritoneal Carcinomatosis through Virus induced Immuno-modulation. Mol. Ther., 2:338-47.
Shmulevitz M, Gujar SA, GyunAhn D, Mohamed, A., Lee PWL. (2012) Reovirus variants with mutations in S1 and L2 genome segments exhibit enhanced virion infectivity and superior oncolysis. J. Virol., 86(13):7403-13.
Previous publications by Dr. Shmulevitz on reovirus oncolysis
Pan D, Pan L, Hill R, Marcato P, Shmulevitz M, Vassilev LT, Lee PWL (2011). Stabilization of p53 enhances reovirus-induced apoptosis and virus spread through p53-dependent NF-kb activation. Br J Cancer, 105(7):1012-22.
Shmulevitz M, Lee PW (2012). Exploring host factors that impact reovirus replication, dissemination, and reovirus-induced cell death in cancer versus normal cells in culture. Methods in Molecular Biology, volume 797, 163-76.
Shmulevitz M, Pan L, Garant K, Pan D, Lee PW (2010). Oncogenic Ras promotes reovirus spread by suppressing IFN-b production through negative regulation of RIG-I signaling. Cancer Research 70(12), 4912-21.
Pan D, Marcato M, Shmulevitz M, Lee PW. (2009). Chapter 26: Host signaling responses to reovirus infection. In: RNA Viruses (Decheng Yang, Editor). World Scientific Publishing Co. Pte. Ltd. pp. 627-654.
Shmulevitz M, Marcato P, Lee PW (2009). Activated Ras signaling significantly enhances reovirus replication and spread. Cancer Gene Ther. 16(4), 382.
Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS. (2008). Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8 dependent mechanism. Blood 15;111(12), 5467-76.
Marcato P*, Shmulevitz M*, Da P, Stoltz D, Lee PW. (2007) Ras transformation mediates reovirus oncolysis by enhancing virus uncoating, progeny infectivity, and apoptosis-dependent release. Mol Ther. 15(8), 1522-30. (*co-first authors contributed equally to this manuscript)
Shmulevitz M, Marcato P, Lee PW (2005). Unshackling the links between reovirus oncolysis, Ras signaling, translational control and cancer. Oncogene. Nov 21;24(52):7720-8.
Marcato P, Shmulevitz M, Lee PW (2005). Connecting reovirus oncolysis and Ras signaling. Cell Cycle. 2005 Apr;4(4):556-9. Epub 2005 Apr 10.
Shmulevitz M, Lee PW (2012). Exploring host factors that impact reovirus replication, dissemination, and reovirus-induced cell death in cancer versus normal cells in culture. Methods in Molecular Biology, volume 797, 163-76.
Shmulevitz M, Pan L, Garant K, Pan D, Lee PW (2010). Oncogenic Ras promotes reovirus spread by suppressing IFN-b production through negative regulation of RIG-I signaling. Cancer Research 70(12), 4912-21.
Pan D, Marcato M, Shmulevitz M, Lee PW. (2009). Chapter 26: Host signaling responses to reovirus infection. In: RNA Viruses (Decheng Yang, Editor). World Scientific Publishing Co. Pte. Ltd. pp. 627-654.
Shmulevitz M, Marcato P, Lee PW (2009). Activated Ras signaling significantly enhances reovirus replication and spread. Cancer Gene Ther. 16(4), 382.
Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS. (2008). Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8 dependent mechanism. Blood 15;111(12), 5467-76.
Marcato P*, Shmulevitz M*, Da P, Stoltz D, Lee PW. (2007) Ras transformation mediates reovirus oncolysis by enhancing virus uncoating, progeny infectivity, and apoptosis-dependent release. Mol Ther. 15(8), 1522-30. (*co-first authors contributed equally to this manuscript)
Shmulevitz M, Marcato P, Lee PW (2005). Unshackling the links between reovirus oncolysis, Ras signaling, translational control and cancer. Oncogene. Nov 21;24(52):7720-8.
Marcato P, Shmulevitz M, Lee PW (2005). Connecting reovirus oncolysis and Ras signaling. Cell Cycle. 2005 Apr;4(4):556-9. Epub 2005 Apr 10.
Other publications by Dr. Shmulevitz
Racine T, Barry C, Roy K, Dawe SJ, Shmulevitz M, Duncan R. (2007). Leaky scanning and scanning-independent ribosome migration on the tricistronic S1mRNA of avian reovirus. J Biol Chem. 282(35), 25613-22.
Wong TA, Fairn GD, Poon PP, Shmulevitz M, McMaster CR, Singer RA, Johnston GC. Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12777-82.
Singh M, Shmulevitz M, Tikoo SK. A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection. Virology. 2005 May 25;336(1):60-9.
Shmulevitz M, Corcoran J, Salsman J, Duncan R. Cell-cell fusion induced by the avian reovirus membrane fusion protein is regulated by protein degradation. J Virol. 2004 Jun;78(11):5996-6004.
Shmulevitz M, Epand RF, Epand RM, Duncan R. Structural and functional properties of an unusual internal fusion peptide in a nonenveloped virus membrane fusion protein. J Virol. 2004 Mar;78(6):2808-18.
Shmulevitz M, Salsman J, Duncan R. Palmitoylation, membrane-proximal basic residues, and transmembrane glycine residues in the reovirus p10 protein are essential for syncytium formation. J Virol. 2003 Sep;77(18):9769-79.
Shmulevitz M, Yameen Z, Dawe S, Shou J, O'Hara D, Holmes I, Duncan R. Sequential partially overlapping gene arrangement in the tricistronic S1 genome segments of avian reovirus and Nelson Bay reovirus: implications for translation initiation. J Virol. 2002 Jan;76(2):609-18.
Shmulevitz M, Duncan R. A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses. EMBO J. 2000 Mar 1;19(5):902-12.
Wong TA, Fairn GD, Poon PP, Shmulevitz M, McMaster CR, Singer RA, Johnston GC. Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12777-82.
Singh M, Shmulevitz M, Tikoo SK. A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection. Virology. 2005 May 25;336(1):60-9.
Shmulevitz M, Corcoran J, Salsman J, Duncan R. Cell-cell fusion induced by the avian reovirus membrane fusion protein is regulated by protein degradation. J Virol. 2004 Jun;78(11):5996-6004.
Shmulevitz M, Epand RF, Epand RM, Duncan R. Structural and functional properties of an unusual internal fusion peptide in a nonenveloped virus membrane fusion protein. J Virol. 2004 Mar;78(6):2808-18.
Shmulevitz M, Salsman J, Duncan R. Palmitoylation, membrane-proximal basic residues, and transmembrane glycine residues in the reovirus p10 protein are essential for syncytium formation. J Virol. 2003 Sep;77(18):9769-79.
Shmulevitz M, Yameen Z, Dawe S, Shou J, O'Hara D, Holmes I, Duncan R. Sequential partially overlapping gene arrangement in the tricistronic S1 genome segments of avian reovirus and Nelson Bay reovirus: implications for translation initiation. J Virol. 2002 Jan;76(2):609-18.
Shmulevitz M, Duncan R. A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses. EMBO J. 2000 Mar 1;19(5):902-12.